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Mouse ESCs carrying translocated chromosomes can be isolated and expanded to establish stable cell lines.
The demonstration that stem cells exist in the adult brain and that they can be isolated and expanded in vitro offers the possibility to test such interesting cells in the paraplegic rat.
Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) have been demonstrated as an attractive cell source for tissue engineering applications because of their ability to be isolated and expanded.
Of note, SOX9+ BCs can also be isolated and expanded from those small airway (~1 mm diameter) samples, which is accessible by open-chest surgery or autopsy but not by bronchoscopy (data not shown).
Human liver-derived stem cells can be isolated and expanded from donated livers unsuitable for transplantation and present a comparable morphology to that of HSCs, which express a-smooth muscle actin, vimentin, fibronectin, CD73, and CD90 in accordance with their mesenchymal origin (Berardis et al., 2014).
We show here that EPCs can be isolated and expanded in vitro under xeno-protein-free conditions.
Similar(47)
After selection on puromycin, single clones were isolated and expanded.
The hNSCs were isolated and expanded according to the protocol described previously70, 71.
We investigated whether these properties are maintained after TC are isolated and expanded in vitro.
Human ADSCs (hADSCs) and rabbit ADSCs (rADSCs) were isolated and expanded.
Chondrocytes were isolated and expanded separately in monolayer cultures and seeded in alginate beads in the presence and absence of hPS of 1% or 10% v/v concentration.
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