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When we scanned the genome with this model, we see that most of our top hits are genes known to be involved in sulfur amino acid biosynthesis (Fig. 6).
The other complexes may be involved in sulfur reduction either directly or indirectly.
However, transcriptional studies of iro in A. ferrooxidans ATCC33020 suggested that it may be involved in sulfur oxidation.
In our analysis of the type strain, iro (AFE2732) was found to be associated with the petII gene cluster thought to be involved in sulfur oxidation [ 46, 47], thus making it unlikely that Iro is the key iron-oxidizing enzyme.
Finally, DsrE family proteins such as that predicted to be encoded by AFE_2556 are small proteins recently shown to be involved in sulfur transfer reactions during sulfur oxidation [ 66].
In S. metallicus, a gene (sor), encoding sulfur oxygenase reductase, which is absent in A. ferrooxidans, is the dominant transcript in sulfur-grown cells and is therefore proposed to be involved in sulfur oxidation [ 51].
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ATP sulfurylases (At3g22890, ASP1; ASP314680, and3; At5g437803780, ASP4) and sulfate transporters (At5g10180, AtSULTR2 1), which are involved in sulfur assimilation and translocation, specifically, are targeted by miR395 [12], [12].
Besides being involved in sulfur mobilization, cysteine desulfurase is proposed to be involved in cellular iron homeostasis [ 16- 18].
Finally, the cloning and phylogenetic analysis of a gene that is involved in sulfur metabolism support the sulfur-oxidizing nature of the Robbea symbionts.
However, β-grasp fold proteins have multiple functions in halophilic archaea, being involved in sulfur chemistry as well as ubiquitin-like protein modification by SAMPylation [ 56].
Six of these are involved in sulfur metabolism including five genes involved in cysteine synthesis (CysC, N, S, K < ZMO003, 4, 7, 0748>, and ZMO0006) and an ABC transport system SsuACB (ZMO1261-3) involved in sulfonate import.
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