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This finding is surprising, because a recent report suggested polo kinase to be involved in polarized transport during oogenesis, where Polo could activate BicD by phosphorylation specifically during oocyte differentiation [6].
In budding yeast, microtubules seem not to be involved in polarized growth [1], [2] and we have verified that cells exiting quiescence in the presence of both Lat-A and nocodazole, a drug that affects microtubule polymerization, were able to form new buds (data not shown).
Mid1p and Fig1p may therefore be involved in polarized cell growth in both organisms.
Rab8 has been shown to be involved in polarized membrane transport and regulation of vesicular transport from the trans-Golgi network (33).
This suggests that the MAP encoded by Os08g40620 may be involved in polarized cellular growth during early steps of CR meristem organization regulated by CRL1.
Here, we speculate about additional machinery (such as the lysosomal calcium sensor synaptotagmin VII and the v-SNARE VAMP7) that could be involved in polarized fusion of lysosomes with the epithelial membrane.
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Finally, Rho1 interacts with the protein Bni1p which has also been implicated in actin organization and is involved in polarized exocytosis [20] [24].
The ventromedial protocerebrum contains the neuropil structure called the lateral accessory lobe [46] that is involved in polarized light signaling [47].
RAB8A is involved in polarized trafficking of vesicles from the trans-Golgi network (63– 67), and is the only RAB-family protein identified in mammalian cilia (68, 69).
One of the mutants displaying a hypo-production phenotype, ΔNCU00600, encodes a protein that is similar to a GTPase, Rho3p, which is involved in polarized secretion in S. cerevisiae [ 33, 34].
To determine whether sie is involved in polarized localization of βH-Spectrin in follicle cells, we stained sie mutant ovaries with an antibody against βH-Spectrin (Thomas and Kiehart, 1994).
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