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The differences observed between live cell and membrane binding may be due to dynamic mechanisms in live cells, as opposed to the static conditions that prevail in purified cell membranes, suggesting that intermediary factors or temporally dependent cellular processes could be involved in enabling peptide interaction with the α7-nAChR.
However, many more chromatin components might be involved in enabling DNA repair and recombination.
Within our cohort, MECOM/EVI1, MLL2, and TET2/IDH are examples of nuclear epigenetic regulators that we suspect may be involved in the pathogenesis of melanoma and could be involved in enabling stem-like characteristics in select subpopulations.
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From the early stages of plant evolution, it seems that strigolactones were involved in enabling plants to modify growth in order to gain advantage in competition with neighboring organisms for limited resources.
The Drosophila homolog of Kirrel3, Kirre is involved in enabling muscle cell fusion events.
Several layers of software are involved in enabling the remote access of the BigBed and BigWig files.
To ask whether PVRL4 is involved in enabling anchorage-independent growth of these cells, we designed four independent shRNAs targeting PVRL4 and stably expressed them in SUM190 cells, confirming the extent of depletion by RT-qPCR.
It is also conceivable that the combined effects of hundreds of chemicals in the environment may be involved in the process of enabling carcinogenesis at the population level, so basic empirical research that can demonstrate carcinogenic effects with minimalistic combinations may initially be needed to reveal the more granular aspects of carcinogenesis.
The immediate impact of this could improve our ability to identify amyloid structures from genomic data alone, to better understand familial mutations that intensify pathogenesis in diseases such as Alzheimer's, to predict the interaction strength of fibril regions that may be involved in nucleation and to enable targeted peptide design to alter fibril structure or inhibit fibril formation.
Furthermore, our knockdown studies show that CASP8 activation depends on the adaptor protein FADD and the autophagy proteins ATG5 and ATG7, suggesting that these proteins may be involved in formation of a complex enabling pro-CASP8 recruitment and activation.
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