In earlier experiments we observed these "mixed fragments" at frequencies between 0.1 and 16% of all obtained reads per run with higher frequencies when more than two samples were involved (data not shown).
Control experiments confirmed that the reaction is insensitive to catalase, implying that H2O2 is not involved (data not shown).
However, when cells expressed the CAPS-associated mutant NLRP3 with the Tet-on system, a cathepsin B inhibitor blocked both cell death and mature IL-1 β release (Supplementary Figure S5c), even though a formation of large particles and lysosomal rupture was not involved (data not shown).
The manufacturer of the BIS monitor (Aspect Medical Systems) had no role in the study design, and will likewise not be involved in data collection, data analysis, or in manuscript preparation.
The funders and sponsors will not be involved in data analysis or reporting; however, they will be provided with copies of any publications arising from the study.
However, their amino-termini contained two conserved aspartic acid residues (Asp-11 and Asp-56) that may serve as phosphate acceptors and their carboxy-termini contained several conserved residues that may be involved in DNA binding (data not shown).
However, little is known about the molecular and cellular basis of these various processes, with the pathogen lipophosphoglycan (LPG), various adhesions, surface and secreted enzymes and toxins all thought to be involved, but existing data are limited when not controversial [ 12, 13, 15, 23- 27].
We found no abnormalities in the expression of E-cadherin, a protein known to be involved in cell-cell adhesion (data not shown) [17].
GPH1 is a glycogen phosphorylase required for the mobilization of glycogen, and YMR315W is an oxidoreductase enzyme thay may be involved in the reduction of farnesol (data not shown).
