The peptide substrate was introduced into single cells, and capillary electrophoresis was used to separate and quantify nonphosphorylated and phosphorylated peptide.
A PKB substrate was introduced into single cells, and the formation of phosphorylated product was quantified at varying times and substrate concentrations.
Peptide VI-B was introduced into single cells by microinjection using finely pulled glass capillaries (FemtoJet Injectman NI 2, Eppendorf, Hauppauge, NY) and an injection pressure of 200 hPa for 0.5 s.
Indeed, with this strategy a variety of genetic variants, either in the same gene or in multiple genes, can be introduced into a single cell line so that they are on the same genetic and epigenetic background (Ding et al., 2013).
While genetic analysis to determine the 1p/19q status may be introduced into routine diagnostic practice, no single test is presently available to indicate tumour progression and poor outcome.
The potential for recombination and reassortment is greatly enhanced in persistent and chronic infections, in which multiple genotypes of a single viral species, or multiple viral species, are introduced into a single host through repeated infections [7], [8].
In this work, a JL channel[6 11] is introduced into the single poly-Si structure.
In this proposed method, following the well-known process of droplet-to-particle formation of pharmaceuticals and ceramics by spray drying and spray pyrolysis, e.g., [43 46], a spray nozzle atomizes the perovskite solution, where the droplets in the form of a mist are introduced into a single- or multi-stage hot wall (tubular) reactor.
Each phage isolate was amplified from one arbitrarily chosen plaque and was introduced into a single exponentially growing PAO1 culture.
To improve overall electrochemical properties of the alloy, especially the discharge capacity, Ce2Ni7-type or LaNi5 secondary phase was introduced into the single-phase alloy by adjusting molar ratio of precursors.
