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Our conclusions are based on the analysis of single cells that avoids bias that might be introduced by sequencing TCRs from the library amplified by the PCR.
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Because false positive SNPs could be introduced by sequence alignment errors and sequencing errors, only the SNPs that were present in more than one EST were used to detect allele-specific transcript structure variation in this paper.
This approach was chosen for its robustness to nonnormality, nonlinearity, and potential outliers that might have been introduced by sequence-specific biases during deep sequencing.
These systematic biases are introduced by sequence divergence in heterologous the species and by other confounding genomic characteristics related to species divergence.
Additionally, three optical fragments (> 2 kb) were not aligned to the K-10 in silico map, most likely due to absence of BsiWI sites on the ATCC 19698 DNA or because artificial BsiWI sites on K-10 that were introduced by sequence errors.
As a matter of fact, variability could be introduced by 454-sequencing [ 39] and PCR amplification; in particular, errors produced in the early PCR cycles could then be spread in multiple reads with a low possibility to distinguish them from real SNCs.
Engineered genes in plasmid or viral vectors under the control of a muscle-specific regulatory sequence may be introduced by intramuscular injection or by the introduction of transfected myoblasts.
When repair templates with the desired changes are present, these sequences may be introduced by gene conversion (Gaj et al. 2013).
The consistency with 1 1 allelic ratio observed in the gDNA data indicates that no allelic bias was introduced by the sequence capture, a critical requirement for this experimental design.
All modifications were introduced by PCR and verified by sequencing.
"Bernstein Collaborations" was introduced by a film sequence of Robbins in 1994 rehearsing with Mr. Woetzel in the rumba solo of "Fancy Free" (the ballet that first brought Robbins and Bernstein together, 50 years before).
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