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The CMAP database can be interrogated with expression change signatures consisting of lists of up and down regulated probe sets.
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It is the opinion of our laboratory that the entire process of molecular transcription and translation be interrogated with validation of global expression profiling results utilizing qRT-PCR followed by proteomic profiling using any combination of Western blotting, immunoperoxidase staining and in situ hybridization.
Gene expression of 24/32 known MGF, 3/4 VEGFs, 13/22 FGFs and 11/19 Wnts could be interrogated with Affymetrix U133 Plus 2.0 arrays (Tables 1A and 1B).
One cell's worth of RNA can now be interrogated with confidence.
In practice, the Connectivity Map database is interrogated with a gene expression signature of interest to ask whether the signature resembles the action of a perturbagen on the reference cells.
After the expression of full-length receptors had been verified by Western blot analysis, their interaction with 12G5 was interrogated with a whole cell ELISA in a 96-well plate format.
Selectivity was interrogated with l-DOPA which gave a flat response of Au-MIP.
The mechanical oscillations of microstructure are interrogated with the aid of fiber-optic Fabry Perot interferometer.
A transcript is interrogated with a set of 16 probes, which is called a "probeset".
The sheep genome database was interrogated with ISGC BLAST using the most current assembly.
Methylation was interrogated with the 8.1 K CpG island microarray (UHN Microarray Center).
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