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For that purpose, we identified the site at which a loop containing the recognition sequence for cre-lox recombinase could be inserted yielding a functional enzyme.
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It does Monsanto no good to figure out which genes from bacteria can be inserted into a crop like corn to help it resist insects, for example, if the genes cannot be moved into a proven, high-yielding strain that farmers are willing to plant.
Moreover, in additional analyses, I transformed the parental support index into a categorical variable to be inserted into the model instead of the ordinally scaled measure yielding to substantially similar results.
Mutant GFP protein was encoded by a gene in which the TAG stop codon was inserted at position 40, yielding mutant 40TAGgfp.
The PCR product was inserted into an EcoRV-digested pJET1.2 (Thermo Scientific) in the opposite direction of Eco47IR (killer gene), yielding pJET-5′-xpp1.
The upstream and downstream sequences of the nprE gene were inserted into plasmid pUK, yielding nprE knockout vector pUNprEK.
PCR products were inserted into vector pcDNA3.1 zeo+), yielding recombinant plasmid pcDNA3.1-huPrP-3AV.
This implanted motif was inserted every 10 seconds, yielding 171 motif-repeat pairs.
Then the LAH-HBc gene was inserted into expression vector, yielding recombinant vector pET28a-LAH-HBc, and the expression was carried out in E. coli BL21 (DE3) strain.
Using the Gateway system (Life Technologies), BMCC1 and PRUNE2 were inserted into pcDNA6.2 (Life Technologies), yielding pcDNA6.2-BMCC1 (340-kDa) and pcDNA6.2-PRUNE2 (30-kDa) expression vectors.
Two unique restriction sites and a copy of the transcription regulatory sequence for ORF6 (TRS6) were inserted between ORFs 1b and 2a, yielding a general purpose expression vector.
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