The restriction site in the 3' UTR into which the IRES-CreER/TetR/FNF cassette was inserted, together with the sequence of ten nucleotides on each side of the insertion sites are as follows.
Another showed packets of ballots apparently just removed from the ballot box, stacked neatly, one on top of the other, an indication that they were inserted together, not individually.
c Both membranes and side holes are inserted together, resulting in the negative phase velocity.
The parallel sliding screws consist of two screws with an integral sleeve which are inserted together: each sleeve is first screwed into and locked to the nail, and then the 7 mm screw is advanced into the femoral head under X-ray control.
The loxP flanked neomycin resistance - thymidin kinase gene (neo-tk) selection cassette was inserted together with a 141 bp wt cDNA fragment of the β1 integrin gene coding for the entire cytoplasmic tail of the β1 integrin chain including the stop codon, into exon 15, just behind and in frame with the transmembrane coding region.
Furthermore, the healing of endosseous implants was not improved when those were inserted together with OCHS [ 21].
Finally, when the three mutations are inserted together, the dynamic range of Y195 is more similar to that of wild-type.
A moist chamber was made in a Petri dish where the cover slip was inserted together with a small plastic vial containing about 1 ml of 2% aqueous osmium tetroxide.
To prevent leaching of agar in the nutrient solution, the bottom of the seed-holders was wrapped with a piece (15 × 40 mm) of grey mesh (1 mm) (Fig. 1c) that stuck to it when both parts were inserted together into the holes of the container cover.
The human point mutation T5M, which leads to a substitution of a threonine by a methionine residue at position 5 of the Cx30 protein, was inserted together with a SphI restriction site via PCR mutagenesis into the corresponding mouse gene, Gjb6 (Fig. 1A and B).
