For that purpose, biofilms were grown on plastic plates set in a Couette–Taylor reactor (CTR) that was inoculated using a "white water" sampled from a paper industry network.
Overnight cultures were inoculated using a single colony from freshly streaked plates in Luria-Bertani (LB) broth.
For each 384-well plate from the library four 96-well blocks were inoculated using a 96-pin replicator.
Six week old WT and + RB tubers were inoculated using a modified whole tuber assay [ 6], as detailed below.
Reactions were inoculated using a 384-pin plastic plate replicator (ISC bio express cat# g32404) directly from the 384-well fosmid glycerol stock (see above).
The cells were inoculated, using a 48-pin replicator (V&P Scientific, VP407AH), onto SC medium lacking leucine and uracil or lacking leucine, uracil, and histidine.
At the age of 6 weeks, plants were inoculated using a solution containing B. cinerea conidia (2 × 10 spores ml−1), 5 mM glucose, and 2.5 mM KH2PO4.
Seedlings used for root infection were inoculated using a mycelium plug (5 mm dia) from the growing edge of a 14 day old colony grown on potato dextrose agar (PDA) at 20°C and with a PDA plug for the controls.
Approx. 1 uL of each saturated culture was inoculated using a 384-pin tool (VP Scientific) in a 20-uL PCR reaction containing 50 ng each of primers T3 and T7 (Stratagene) and 0.5 U Taq Polymerase (GE Healthcare).
For pre-cultures, 5 ml of LB medium was inoculated using a single colony picked directly from agar plates, and then cultivated at 37°C with shaking at 180 rpm for 12 h.
