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The country's "current vaccine development and production capacity is severely lacking," the report notes, adding that "the country has not developed or adequately tested a system that will ensure that all Americans would be able to be inoculated in a short period of time".
The 23-page report further warns that the country's "current vaccine development and production capacity is severely lacking," adding that "the country has not developed or adequately tested a system that will ensure that all Americans would be able to be inoculated in a short period of time".
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Prior to DNA extraction, the genetically engineered, cognate reference strain P. fluorescens CHA0/c2 was inoculated in a reference soil.
In some cases the flasks were inoculated in a time-displaced way to collect samples of all cultivation phases.
T. rubrum and T. mentagrophytes were inoculated in a preliminarily melted and tempered to 45 48 °C PDA medium.
Every single mutant clone was inoculated in a 96-deep well plate and cultivated for 20 h in 800 µl of LB medium with 20 g/L glucose.
All shake flasks were inoculated in a shake incubator chamber (Multitron II, HT Infors, Bottmingen, Switzerland) at 30 °C and 120 rpm.
One PGPM was inoculated in a 15N-labelled (NH4 2SO4 + DMPP depot in rhizobox-grown maize (Zea mays L). and root colonization was measured.
An inoculum from a culture of isolate RH5 was inoculated in a tube containing MRVP (methyl red Voges Proskauer) broth and incubated for 24 h at 35 °C.
Then, the T. viride was inoculated in a liquid SDA media and grown aerobically at 35°C and agitated at 150 rpm on orbital shaker.
BtCry2Ab was inoculated in a 5-ml Loria broth (LB) culture tube and incubated overnight at 150 rpm shaking at 37 °C.
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