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The supramolecular hydrogels are thixotropic and reversible, and can be injected through a needle from a syringe to form injectable drug delivery systems.
In particular, the thermogel is soft enough to be injected through a 21-gauge syringe needle even as a gel state.
In this study, we created cell-adhesive and degradable gelatin scaffolds that could be injected through a conventional needle while maintaining a predefined geometry and architecture.
They could easily be injected through a 26-gauge needle at 4 °C, and were capable of gelling within 6 s at 37 °C to form highly flexible gels with moduli matching those of the rat and human myocardium.
Anesthetized animals were placed on stereotactic frame, and 104 9LGS cells were suspended in 1 μL culture medium with antibiotics before to be injected through a burr hole in the right caudate nucleus (3.5 mm lateral to the bregma, 6 mm below the skull surface).
Additionally, the delivery matrix is a fluidic suspension that can be injected through a 25 gauge needle enabling minimally invasive delivery.
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CO2 was injected through a single injection well, which was surrounded by four monitoring wells.
Vaporized hexamethyldisilazane (HMDSN) was injected through a central injection probe, while nitrogen or hydrogen gases were added through the torches to the argon plasma.
In order to promote the liquid spray dispersion in the flow, the kerosene/air mixture is injected through a swirling injection system.
A single dose of pilocarpine hydrochloride (1.2 mg/ μL, total volume 2 μL; Sigma-Aldrich, USA) was injected through a needle connected to an injection pump (flow rate: 1 μL/min; Stoelting Co., IL, USA).
Neutralizing antibody against MCP-1 or KC (R&D Systems, Minneapolis, MN, USA) was injected through a tail vein 1 h after injection of LPS.
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