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Fluid replacement must be initially performed with crystalloid solutions, while use of colloids should be restricted to cases of unresponsive shock [ 66].
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Hence, the qPCR was initially performed with primers which recognize the common upstream promoter regions of the 3 main ups subtypes.
To ensure that all the reference MAbs were able to bind their target protein, immunoprecipitations were initially performed with MAbs incubated with purified virions solubilized with Triton X-100.
Immunoblotting was initially performed with anti-OBP1 antibody at a 1∶800 dilution and a 1∶1,000 anti-guinea pig secondary antibody (Jackson ImmunoResearch Laboratories, Inc ., using the Amersham ECL Western Blotting detection reagents (GE Healthcare) or Pierce SuperSignal West Pico chemiluminescent substrate (ThermoScientific).
All simulations were initially performed with full occupancy for bound ligands and ions.
All analyses were initially performed with sex of participants as a between-subjects' factor.
Sequence alignments were initially performed with ClustalW [ 34] in BioEdit version 6.0.6 [ 35] and adjusted manually.
The present study was initially performed with tritium-labeled benzene, administered by IP injection.
A descriptive analysis was initially performed with separate datafrom the state capitals and other cities.
Genotyping was initially performed with the ABI PRISM 7700 (TaqMan) sequence detection system (Applied Biosystems, UK).
Genotyping on 1543 individuals was initially performed with the Illumina HumanHap 300K BeadChip for 1568 blood-derived DNA samples.
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