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They require the disulfide bond to be initially cleaved with reducing agents to afford the two free cysteines to which the bridging reagent is then added.
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In AD, Aβ peptides are generated by successive proteolysis of APP that is initially cleaved by β-secretase, and subsequently by γ-secretase [ 31- 33].
The majority of APP molecules in non-neuronal cells are initially cleaved by α-secretase between positions 16 and 17 of the Aβ domain.
On the other hand, the C-terminal arginine 117 residue of newly synthesized Atg8 is initially cleaved by the Atg4 in order to expose a glycine residue [ 11].
The Boc group was later cleaved with 4 M HCl in dioxane.
Cells were then cleaved with TrypLE and counted using a hemocytometer, as described earlier.
This fragment was cloned into BTM116-R-Ras that had been cleaved with BamHI, blunted with Klenow fragment and cleaved with SacII.
Human lamin A is initially produced with a CaaX box (designated pre-lamin A) that is modified and subsequently cleaved to produce mature lamin A lacking a CaaX box [3].
The radiolabeled unmodified substrate was cleaved to a similar extent, regardless of whether unmodified substrate or γ-HOPdG-modified substrate was initially incubated with the enzyme.
After trx-PT-Fc was cleaved with recombinant enterokinase, PT-Fc was obtained.
For NubG fusions, pNXgate32/33-3HA or pXNgate21-3HA vectors were cleaved with EcoRI/SmaI, whereas pMetYCgate vector was cleaved with PstI/HindIII.
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