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Compared to the plain liposomes and PEGylated liposomes, the targeted folic acid functionalised liposomes exhibited enhanced cellular uptake by the FA receptor positive SKOV3 cells, but not the negative MCF7 cells, and this enhanced uptake could be inhibited by adding excess free folic acid, indicating high specificity of FA ligand-receptor endocytosis.
The labeling of run-on transcription sites could be inhibited by adding α-amanitin (Sigma) at a concentration of 100 µg/ml.
Figure 3 shows that the hydrolysis of meropenem can be inhibited by adding NDM-1 inhibitors to the cells.
For one T-cell specificity, moreover, we demonstrated the specific killing of a melanoma cell line that could be inhibited by adding cold target cells.
On average, 25.9 ± 34.9 iRBCs bound to 100 mock‐transfected CHO‐745 cells, which could not be inhibited by adding any of the antibodies to the endothelial surface proteins studied.
In isolated liver mitochondria, which lack both CK and bound HK2 to stabilize contact sites, cytochrome c release can be inhibited by adding dextran to mimic the presence of cytosolic proteins that usually maintain osmotic equilibrium across the OMM (Doran and Halestrap, 2000).
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Fungal growth is inhibited by adding 0.2 g Cycloheximide.
RNA degradation in a crude extract of Zn-deficient rice shoots was also higher than that in control shoots (Figure 4a), and it was inhibited by adding ZnSO4 but not FeSO4 (Figure 4b).
Cells were incubated at 37°C in 5% CO2 for 1 min. Then, the flasks containing the cells were gently tapped to aid cells segregation and observed under inverted microscope (if cells segregation is not satisfying, the cells will be incubated for another minute) Trypsin activity was inhibited by adding 5 ml of fresh complete media (10% FBS).
Release of cytokines was inhibited by adding 1 µg/ml brefeldine A (Sigma) 4 hours before the end of stimulation.
Lysosomes were inhibited by adding chloroquine to a final concentration of 50 µM 30 min [45], [46] before addition of cycloheximide and were present throughout the experiment.
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