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Though enzymatic function can be inferred by sequence and structure similarity, the relations between sequence, structure and function are far from being completely understood.
As the catalytic residues of Kid are known to be R73 and D75 (Kamphuis, Monti, van den Heuvel, Lopez-Villarejo, et al. 2007), the E78 residue could be inferred by sequence similarity D75.
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Then, the molecular functions of the genes are inferred by sequence analysis and database searching.
The putative function of identified proteins was inferred by sequence homology either from the automated blastp search provided by Joint Genome Institute [ 26] or after manual curation of gene models.
The putative function of identified proteins was inferred by sequence homology either from the automated blastp search provided by Joint Genome Institute [ 2] or from a manual blastp search provided by NCBI.
In addition, if a protein is assigned an incorrect function in a database, the error could carry over to other proteins for which functions are inferred by sequence relationship to the protein with errant function assignment [ 12- 14].
The identities of phage-displayed peptides were inferred by sequencing the library-specific DNA inserts amplified by PCR from the T7 phage display vector.
This conversion allows the position of methylated cytosines to be inferred by traditional sequence alignment procedures as described in a highlights talk by Martin Frith [ 18] (AIST).
The underlying process of the electric charge evolution of the PGI proteins can be inferred by ML sequence reconstructions [ 34] based on the recent ray-finned fish phylogeny [ 18].
Since HIV evolves rapidly, viral sequences isolated from infected individuals are typically unique, and transmission networks can be inferred by identifying sequences that are genetically closely related (Smith et al., 2009, Wertheim et al., 2013).
The bona fide sites can then be inferred by multiple mapped sequenced fragments.
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