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Besides the strong inducer IFNγ, the GBPs can also be induced by type I interferons [ 2- 6], tumor-necrosis-factor α (TNF-α), interleukin-1β (IL-1β), IL-1α [ 7, 8], and TLR agonists [ 5].
To determine whether the expression of PD-L1 can be induced by type I IFN in B16F10 cells, we treated B16F10 cells with IFN-β and the flow cytometry results showed that IFN-β can induce PD-L1 in a concentration-dependent manner (Fig. S3A).
This gene is also important here because it is well-established to be induced by type 1 interferons [20].
By using these criteria, 22 genes were significantly up-regulated, 9 of which are known to be induced by type I interferon (Table 1).
Furthermore, salivary epithelial cells from Sjögren's syndrome patients can be induced by type I interferon to express BAFF [ 57].
We currently have no explanation for this observation as expression of ISG20 is known to be induced by type I IFNs [ 26, 27].
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The list of decreased genes contains 11 genes whose expression has been reported to be induced by type-1 interferon.
The results obtained in this study suggest that immediate-type allergic reactions after vaccination in dogs were induced by type I hypersensitivity mediated by IgE directed to vaccine components.
Whether the transcription of all genes involved in cellular attraction and migration of DC is induced by type I interferons remains to be established.
To investigate whether the expression of feline Tetherin/BST-2 is induced by type I IFN, we treated CRFK cells with 100 or 1,000 units/ml of IFN-α A/D for 24 h and determined the level of feline Tetherin/BST-2 mRNA by quantitative real-time RT-PCR.
In addition, we showed that the expression of feline Tetherin/BST-2 was induced by type I IFN similar to human Tetherin/BST-2 (Figure 2), suggesting that Tetherin/BST-2 functions as a host innate antiviral system against a wide variety of viruses.
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