Pathway activation was induced by constructing a cell line with stable expression of Wnt1.
(3) Appendicitis is induced by constructing an appendiceal pouch from the caecal lymphoid patch, which is the equivalent of the human appendix.This appendiceal pouch is obstructed by rubber band ligation using standardised negative aspiration.
We found also in PBs proteome 12 proteins classified into stress related proteins (see Additional file 2: Table S2) and according to transcriptomic data found in the literature, half of them are likely to be induced by the construct because their trancript are almost not detectable in leaves grown in normal conditions.
Structural models of IgEDNP FcεRI aggregates that may be induced by DF3 were constructed on the basis of available structural knowledge, such as information about the molecular basis for the high-affinity interaction of IgE with the FcεRIα ectodomain.
For expression in S. cerevisiae, an inducible expression vector pYES2/eGFP (pYES2/CT with a C-terminal eGFP) was used whereby fusion constructs could be induced by the addition of 4% (w/v) galactose.
Similarly, Akt S473 and Erk phosphorylation were induced by both adenoviral constructs, with a concomitant increase in higher molecular weight forms of PARP.
Although biopsies, taken 4 months after reconstructing the intra-oral bone defect, showed bone formation in 3 patients, only in 1 patient bone formation was induced by the tissue-engineered construct.
To this end, human telomerase reverse transcriptase (hTERT) targeting trans-splicing ribozymes whose expression and activity were induced by HRE under hypoxia were constructed.
The changes in the color and fluorescence are ascribed to the ring-opening of spirolactam in rhodamine fluorophore, which is induced by a binding of the constructed receptor to Hg2+ with the association and dissociation constants of 0.22 × 105 M−1 and 25.2 μM, respectively.
The expression of the fusion construct was induced by adding IPTG to a final concentration of 2 mM for 2 hours.
