Since c-Rel is required for the expression of Bcl-X, c-myc, and other survival molecules in Wehi-231 cells [76], [77], [79], [80], [87], [88], the lower percentage of GFP+ cells in the c-Rel-siRNA samples may be indicative of cell apoptosis induced by the expression of the specific siRNA.
These conflicting trends may be indicative of cell type differences or tumor stage.
However, by 16 hours expression levels had risen dramatically, which may be indicative of cell cycle arrest prior to apoptosis.
Most of these Ppα-DOX expressing cells also showed staining with the fluorescent dye solophenyl flavine 7GFE 500, suggesting changes in the cell walls which could be indicative of cell wall reinforcement [ 26].
However, the addition of higher concentrations of ferric iron to the culture medium did not have the same effect, and may be indicative of cell damage due to iron overload.
The right shift in the 2N peak becomes a shoulder in 7 hour and 9 hour samples and is indicative of cells in S phase of the cycle.
Because EpCAM is not expressed in the stroma or in the MM, the NCAM+EpCAM− subpopulation is indicative of cells originating from both of these areas, while NCAM+EpCAM+ cells are a heterogeneous pool of progenitor cells from the nephrogenic zone, including newly developed tubules.
Caspase 3 is a downstream effector protein of apoptosis and its elevated activity is indicative of cells undergoing apoptosis.
The expression of pluripotent markers is indicative of cells that have the capacity to differentiate into cell types derived from all three germ layers.
T47D cells, in contrast, did not show bundles but did show chromatin condensation, which is indicative of cells arrested in mitosis.
