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Interestingly, it has been demonstrated that the half-life of antibodies recognizing the IL-6 receptor [ 3] or proprotein convertase subtilisin kexin type 9 (PCSK9) [ 4] can be increased by engineering these antibodies for decreased antigen-binding at acidic pH.
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Moreover, the stability of T. reesei endo-1,4-β-xylanases II (XynII) was increased by engineering a disulfide bridge at its N-terminal region.
Although the enzymes' S/H ratio can be increased by protein engineering, such approaches require a suitable screening assay.
We employed RMCE to test if expression from a weak integration site can be increased by chromosomal engineering of the integration site.
But road congestion could be increased by nearly 1,000 engineering projects being carried out across Britain's rail network, meaning some lines will be closed.
… When the conference committee convenes, the House position will be that SBE must be reduced by $15.35 million, and the four physical sciences and engineering directorates will be increased by $15.35 million".
Antibody efficacy has been increased by genetic engineering to humanize the antibodies and to increase their effector functions including antibody dependent cellular cytotoxicity.
TIAs production in plants is generally low and difficult to synthesize chemically, limiting the utility of these therapeutic chemicals unless production is increased by metabolic engineering [ 11].
Either targeted overexpression of ScGal2 improved L-arabinose utilization [ 27] or expression of GAL2 was increased by evolutionary engineering of a yeast strain for improved fermentation of L-arabinose [ 37].
For example, the overall protein synthetic capacity of E. coli extracts has been increased by genetically engineering pathways responsible for recycling key amino acid precursor molecules, leading to the development of the E. coli strain KGK10 (Calhoun and Swartz, 2006; Knapp et al., 2007; Michel-Reydellet et al., 2004).
The potential of these biocompatible scaffolds for tissue engineering is increased by preserving porous matrix integrity, promoting angiogenesis and reducing foreign body reactions.
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