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While BSA appeared to be in a ordered end-on position occupying about 14 nm2 at maximal adsorption (Table 2) under conditions of moderate electrostatic attraction between BSA and silica/DODAB (1 mM NaCl, pH 6.3) further increasing this attraction drove massive BSA adsorption onto the particle as detected from the small area occupied per protein on the silica/DODAB surface, about 3 nm2 (Table 2).
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