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Until now many researchers have argued that it would be impossible to sequence the entire genome of an extinct creature because the samples are so degraded.
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Before multiple parallel sequencing was developed, it was impossible to sequence multiple times the genome of the same patient.
In some cases, the underlying mutations can not be found at all, because it is impossible to sequence complete genes routinely.
Since it is impossible to sequence the complete CFTR gene routinely, clinical laboratories must rely on test systems that screen for a panel of the most frequent mutations causing disease in a high percentage of patients.
The true core- and pan-genome sizes, here denoted γ and η respectively, will most likely remain unknown for any species, since it is impossible to sequence and annotate all existing strains.
These samples were sequenced with the MY 09/11 primers; five had either degenerated in storage or would not amplify, two contained multiple templates and were impossible to sequence, while four were successfully sequenced and identified but none were infected with novel types, merely types not included in the ELISA probe cocktail (Table 2).
After all these years, it may be impossible to trace the sequence of facials, spa treatments, mud baths, cosmetic procedures, lifts and staples, collagen implants, outpatient touch-ups, tannings, Botox injections, cyst and growth removals, and stem-cell injections that have caused a 72-year-old man to have the face of a 9-year-old Filipino girl".
However, if identical mtDNA haplotypes are shared among many ethnic groups from different parts of Africa, it would be impossible to use DNA sequence information to determine which single ethnic group was the source of a particular maternal ancestor.
Next-generation sequencing (NGS) technologies such as Illumina's Genome Analyzer and Roche's 454 sequencing platforms have opened the way to tackle sequencing of large genomes like those of barley and wheat which would be impossible to address by Sanger sequencing [ 16].
The vast amounts of data produced by deep sequencing can be impossible to analyze and visualize due to high storage, memory and screen size requirements.
Sequencing platforms employing shorter reads may pose difficulty in the accurate assembly of repetitive regions because it may be impossible to place reads that lack flanking sequences, which should be distinct for a locus, in a single location with 100% certainty (Treangen and Salzberg, 2012).
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