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Flexibility is linked to creativity and is important for functioning in a changeable world.
Protonation of a buried water cluster has been shown to be important for functions of bacteriorhodopsin as well (Garczarek et al., 2005).
However, conformational variability is seen around Leu1706 which is a hot spot for cardiomyopathy mutations suggesting that this might be important for function.
While three-dimensional conformation may be important for function, it is not necessary for definition.
This conformational variability is likely to be important for function although there is no evidence that zinc is involved in vivo.
In mammalian systems, αPIX has been shown to be important for function of the immune system [79], but βPIX knockout has not been reported.
Finally, the conformational differences in the β4/β5 and β7/β8 loops of the two independent Spy0129 molecules point to a flexibility that may be important for function.
This glycosylation may be important for function.
We speculate that rescue is incomplete because PAK-1 functions in a multiprotein complex, where stoichiometry may be important for function.
One can expect that the longer exons are more likely to retain their functionality after shuffling so they are important for function and maintained in evolution.
An increasing number of enzyme systems show the presence of conserved protein motions that are important for function.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com