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Then, the cells were immediately analyzed via flow cytometry, and the data were calculated using a FACSCalibur flow cytometer and CellQuest software (BD Biosciences).
Light intensity can be calculated using this formula.
Coverages were also calculated using the Genome Analysis Toolkit.
MAGGIC score was calculated using online calculator.
The post-TMS MMN responses were calculated using Method 1 only, because only oddball sequences were presented immediately after TMS.
Scores were calculated using equal weights.
Changes from baseline scores were calculated, using a 1-s baseline interval immediately preceding either the selection or the feedback phase.
With the exception of wavelet model thresholds, all alarm thresholds were calculated using the six years of visit data immediately preceding the prediction day.
The arrays were washed and immediately scanned using GenePiX 4000B array scanner (Axon Instruments, Foster City, CA, USA) and fluorescence ratios (tumor/reference) were calculated using GenePix software.
The eGFR was calculated using the Modification of Diet in Renal Disease formula based on creatinine levels immediately before amputation, sex, and race (11).
Averages were calculated using three spike-ins.
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