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Depending on the oral contrast agent used and preference of the radiologist, the small bowel can then be imaged using either fluoroscopy, CT or MRI.
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In each case the paintings were imaged using either both the VNIR and SWIR hyperspectral imaging cameras or just the SWIR camera.
The topography of the washed interstitial biofilms was imaged using either tapping mode atomic force microscopy (AFM) or 3D optical profilometry.
Actin contractility in bulk was imaged using either a 20 × air objective (NA = 0.6) or a 40 × oil objective (NA = 1.3).
The slices were imaged using either an epifluorescence microscope (Nikon TE300, Nikon) or a confocal microscope (Leica TCS-SP5, Leica) equipped with a × 63 1.40 NA oil-immersion objective.
EGFP and mWasabi were imaged using either a standard EGFP filter set (41017, Chroma), a QuantaMaxTM Green set (Omega), or a BrightLine GFP set (3035B, Semrock).
Live-cell time series and fixed cell preparations were imaged using either an inverted LSM 410 or 510 Meta confocal microscope (Zeiss) equipped with 100× or 63× Neofluar 1.3 oil-immersion objectives.
The preparations were mounted in glycerol for analysis, and the posterior midgut was imaged using either a Zeiss Axioplan stereo microscope equipped with Nomarski optics or an Olympus BX53 phase contrast microscope equipped with a 4×/0.13 UPlanFLN lens through CellSens software (Olympus, Japan).
Neuromelanin can be imaged using MRI.
The samples were imaged using our FMM system with either objectives UPLSAPO 10x/0.40 numerical aperture (NA) or LUCPLANFLN 20x/0.45 NA to evaluate our FMM system in term of image quality improvement.
Finally, the top glass slide was peeled off and the contact area of either slide was imaged using an Ultrasharp AFM probe (NSC21/Ti Pt, MikroMasch) operated in tapping mode.
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