Therefore, tracking was essential in order for oocytes to be imaged continuously over a period of 12 hours.
To verify that the observed myelin retraction and splitting was due to repetitive stimulation, control samples were imaged continuously at a rate of one frame per second and an interval of 20 s for 15 min without stimulation (Fig. 2H).
Although only plate #1 could be imaged continuously, selected plates from other positions were photographed at the end of the experiment as part of the astronaut harvest operations on orbit.
The skin was imaged continuously from 1 to 15 min after injection.
We compared cultures starting at P + 20%, P + 40% and P + 55% that were imaged continuously for up to 20 hr each (Video 2); together these time intervals cover the development from layer selection to synapse formation over a 50 hr time period.
Only the bottom tier plate in position 1 of the GIS was imaged continuously, but representative plates from other positions in the ABRS/GIS were photographed prior to harvest at the maturation of the experiment.
Following ten minutes of rest, endothelium-independent responses were examined by administration of a 500-μg sublingual glyceryl trinitrate tablet (Alpharma, Barnstaple, UK) while the brachial artery was imaged continuously for five minutes.
Following ten minutes of rest, endothelium-independent responses were examined by administration of 500 microgram sublingual glyceryl-trinitrate (GTN) tablet (Alpharma, Barnstaple, UK) while the brachial artery was imaged continuously for five minutes.
Intracellular calcium dynamics of 15 80 spontaneously active PNs were imaged continuously within a 250 µm by 50 100 µm wide region for 5 20 min with a temporal resolution Δt = ∼250 ms. For our experiments, we chose YC2.60 over related GECIs, such as D3cpv and YC3.60, for the following reasons.
SPIO and IgG-SPIO treated groups were imaged before injection, continuously for 4 hours after injection, and again at 24 hours.
