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Previous work showed that natural products produced by Myxococcus spp. could be rapidly mapped to producing strains and novel natural products could be identified using secondary metabolomics.
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Potentially chimeric sequences were identified using secondary structure predictions, the Chimera_Check command version 2.7 provided by the Ribosomal database project [52], and partial treeing analyses [53].
These target gene sequences can be identified using RNA secondary structure analysis software (e.g., RNAfold, MirScan, and MFold) combined with dynamic analysis [ 35].
After washing, protein complexes were identified using appropriate secondary horseradish peroxidase-coupled antiserum.
The initial comparative modeling templates were identified using the secondary structure enhanced profile-profile threading alignment (LOMETS) and the four part iterative threading assembly refinement protocol of I-TASSER [38] [39].
When the SEM electron beam was engaged, the area of interest was identified using the secondary electron detector in a wide field.
The risk factors for non-adherence to ARVs were identified using the secondary definition, which included attending the referral HC and having a new diagnosis of HIV infection during the current pregnancy.
IHD was identified using primary and secondary diagnoses from the International Classification of Diseases (ICD), revisions 9 and 10 (codes 410 414 in ICD-9 and I20-I25 in ICD-10) in the Swedinh Hospithe RegiSwedish Hospital Registry and/Outpatientf Death Registry.
Remaining tRNAs were identified using alignment and inferred secondary structures (Additional file 3).
Using the administrative data, coexisting medical conditions were identified using all primary and secondary discharge diagnoses recorded in all hospital discharge abstracts within one year prior to registration[ 13].
Infants with NEC diagnoses were identified using a primary or secondary ICD-9-CM code of 777.50-53 in the hospital or physician claims at the time of initial hospitalization.
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