Aptamers, short RNA or DNA molecules that bind distinct targets with high affinity and specificity, can be identified using high-throughput systematic evolution of ligands by exponential enrichment (HT-SELEX), but scalable analytic tools for understanding sequence-function relationships from diverse HT-SELEX data are not available.
High grade AIN (HGAIN) can be identified using high-resolution anoscopy (HRA) or colposcopy of the anus and perianus after application of 3-5% acetic acid.
Strong, recent selective sweeps causing the fixation of large haplotypes may be identified using high-density SNP panels, however, older sweeps which have accumulated new mutations and weak sweeps which have resulted in the fixation of relatively small haplotypes will not be detected.
The evolving microstructural features are identified using high resolution scanning electron microscopy and energy dispersive spectroscopy and compared with isothermal testing in pure oxidising conditions.
Candidate markers were identified using high throughput sequencing technology (GS-FLX) on DNA extracted from fossil moa bone and eggshell.
They are all holometabolan insects and only recently, small RNAs have been identified using high throughput sequencing in the migratory locust (Locusta migratoria) [20] and in the pea aphid (Acyrthosiphon pisum) [21], which are hemimetabolan species.
Markers of red propolis quality were identified using high performance liquid chromatography (HPLC) coupled to an ultraviolet detector (Shimadzu).
The first set (set1) comprised 22 putative variant positions, which had been identified using high resolution melting curve analysis (Wittwer et al., 2003) and then veryfied by genotyping in every individual using a MALDI (Matrix-Assisted-Laser-Desorption/Ionization) TOF (time of flight) mass spectrometer (MassArray® system, Sequenom Inc., San Diego, USA) for SNP detection.
