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The terminator base can be identified using fluorescence labeling, isotope labeling, mass labeling for mass spectrometry and measuring enzymatic activity using an enzyme conjugate.
After two washes (PBS-albumin, 300 g, 10 min, 25°C), and fixation (2% paraformaldehyde in PBS, 30 min) the levels of phagocytosis were revealed by the florescent intensity of cell-engulfed beads, and could be identified using fluorescence flow cytometry (FACScalibur, BD Biosciences).
HER2 and basal-like subtypes can already be identified using fluorescence in situ hybridization and immunostaining for ER, PR and HER2.
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Thresholds of positivity were identified using fluorescence minus one controls for each color on cells from each sample group.
The positions of these templates were identified using fluorescence imaging of the DNA-specific dyes.
The founder fish were out-crossed with wild-type fish, and the germline mutations were identified using fluorescence PCR (Sood et al., 2013) and sequencing.
Acute slices from wildtype animals were prepared 3 days after retroviral infection and GFP-expressing 3 day old cells were identified using fluorescence microscopy and recorded using whole-cell voltage clamp techniques.
The 24,XY cells were identified using fluorescence microscopy, each disomic sperm was scraped off the slide using a glass needle attached to a micromanipulator and then put into a PCR well.
Cell boundaries were identified using YFP fluorescence and total alexa 555 fluorescence per cell was quantified using ImageJ software.
Here, similar to Figure 3B, infected cells were identified using EGFP fluorescence (Fig. 4C, top images), whereas red fluorescence indicated incorporation of Cy3-labelled antibodies against cleaved caspase 3 (Fig. 4C, centre images).
The three-dimensional pore structures in waste activated sludge floc were identified using the fluorescence in situ hybridization (FISH) and confocal laser scanning microscope (CLSM) images.
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