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Besides, a single-tube multiplex PCR, which is a cost-effective version of dual-priming-oligonucleotide based system, was designed for two common mutations of α-thalassemia in China (Hb Constant Spring and Hb Quong Sze), and these two mutations can be identified in samples by use of dissociation curve analysis.
According to these cut-off values, 7% mutant tumor cells (or 3.5% mutant alleles) would reliably be identified in samples with wild-type DNA Cts <33, a condition achieved in 95% diagnostic samples and in 1/3 of the very fragmented samples in group B (Table 1).
This high sensitivity may allow fusions to be identified in samples with low tumour purity or in samples in which a particular fusion is only present in a clone which is a small proportion of tumour cells.
Because FMR1 mRNA stability may be related to the size of the CGG repeat within its UTR and the pathology of FMR1 related disorders [ 28], in the following sections we have established a method to normalize for mRNA degradation independent of the CGG expansion size, so that the clinical relevance of the CGG related FMR1 mRNA toxicity can be identified in samples with variable rRNA quality.
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We examined whether the SNPs could be identified in sample pools that were two or three times as large.
Mytilus edulis alleles were identified in samples from all 44 sites.
Mytilus trossulus alleles were identified in samples from 6 sites in the west and south-west of Scotland.
The palynological occurrences that were identified in samples provided a possible stratigraphical age for the Erlian Formation.
Although bacterial species in leachate and bulk samples varied with sample collection date, eight species were identified in samples from multiple sampling dates.
Copper-based green pigments were identified in samples, malachite and atacamite, are the principal green pigments widely found in ancient wall paintings [19 21].
The presence of A/H5N1 virus was identified in samples collected in poultry and submitted from 9 of these countries.
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