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Thus, methylcytosines could be identified by sequencing of bisulfite PCR clones (Materials and Methods).
The virus species in the positive samples could be identified by sequencing.
HIV resistance mutations to antiretrovirals will be identified by sequencing of the pol genes.
Cellular organisms can be identified by sequencing the 16S rRNA genes in the sample [ 37- 40], though this misses viruses.
The main shortcoming of molecular testing is that mutations must be identified by sequencing the gene because they are heterogeneous.
IDH1- R132H mutation accounts for nearly 90% of all IDH mutations and can be demonstrated by immunohistochemistry, although other mutations can only be identified by sequencing [ 11, 13].
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UL54 and UL97 gene mutations were identified by sequencing.
ORFs determined to express soluble recombinant proteins were identified by sequencing and analysed by small-scale IMAC and SDS PAGE.
Since U1 transcripts with both long and short poly(A) tails were identified by sequencing of RLM-RACE products (Fig. 4c and Supplementary Fig. 4), aberrant polyadenylation may involve both canonical nuclear poly(A) polymerase and the TRAMP complex.
The selected candidate was identified by sequencing the 16S rRNA.
Positive transformants were identified by sequencing (Baseclear, Leiden, The Netherlands).
More suggestions(19)
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