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Each test pool of Cy3-labeled transcripts will be hybridized in triplicate against reference pool 4 of Cy5-labeled transcripts.
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Three animals per group were used in the immunoprecipitation microarray experiments, and microarrays were hybridized in triplicate for each sample.
Each condition was hybridized in triplicate.
To assess the ubiquity and specificity of the oligonucleotide probes, the DNA microarray was hybridized in triplicate with genomic DNA isolated from either L. infantum or L. major.
Total RNA from skeletal muscles collected from broilers and layers at E10, E12, E14 and E18, and prepared as described above, was hybridized in triplicate with three biological repeats from broilers and layers at each developmental stage.
Each sample was hybridized in triplicates to Illumina Sentrix HumanHT-12 BeadChip gene arrays (Illumina, San Diego, USA).
All profiles were generated in triplicate biological replicates except DMSO-treated samples, which were hybridized in octuplicate since they were used as internal controls for each timepoint.
For one-color platforms, each pool of targets will be hybridized separately in triplicate against the test arrays.
Two different samples were prepared and hybridized in triplicate to Affymetric GeneChips; these are called the 'constant' (C) and 'spike' (S) samples.
The aRNAs were mixed in equal amounts, with the fluorescent cyanine dyes Cy3 and Cy5, and hybridized in triplicate to the microarray.
To determine minimum number of viable CD138+ plasma cells necessary to generate a high quality GEP and reproducible GEP70 score, we performed two titration studies in which varying amounts of pooled MM aRNA were hybridized to Affymetrix microarrays in triplicate.
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