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The new vaccine is to be grown in human cells suspended in large tanks known as bioreactors, and will be purer.
Studying the parasite in the lab is challenging because it must be grown in human red blood cells, and it remains virtually impossible to study the parasite during infection in the human bloodstream.
The viral E1 gene is deleted from the vector genome, thus the virus cannot replicate and can only be grown in human embryonic kidney cells that are transformed with the viral E1 gene.
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Both P. falciparum and P. vivax have been grown in human liver cells; partial development of P. ovale in human liver cells was achieved; and P. malariae was grown in chimpanzee and monkey liver cells.
Viruses were grown in human melanoma MeWo cell line.
P. falciparum parasites were grown in human A+ erythrocytes as previously described [29].
Parasites were grown in human foreskin fibroblasts (HFF, which were kindly provided by David Roos (University Pennsylvania, see ref. [83]).
RH strain T. gondii were grown in human fibroblasts in DME media with 10% fetal calf serum and 1% Penicillin Streptomycin (GIBCO-BRL) in a 5% CO2 incubator.
The parasites were grown in human erythrocytes (O+), RPMI 1640 medium supplemented with 25 mM HEPES, 20 mM sodium bicarbonate and 0.5% AlbuMAX II (Invitrogen, Germany) at 4% hematocrit.
To identify genes that were up or down regulated in response to growth in AF, GAS was grown in human AF or standard laboratory media (THY) and samples for expression microarray analysis were collected during mid-logarithmic, late-logarithmic, and stationary growth phases.
Parasites were grown in human erythrocytes in RPMI 1640 medium (Invitrogen Singapore Pte Ltd ,Singapore) supplemented with 0.5% Albumax I (Invitrogen), 2 mM L-glutamine, 50 µg/ml hypoxanthine and 25 µg/ml gentamycin at 2.5% hematocrit with a gaseous phase of 5% CO2, 3% O2, and 92% N2 at 37°C.
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