All secondary immunizations were given the respective amount of PA in PBS i.p.
18 As most of the short courses of intravenous steroids are given in secondary care, it is not inconceivable that these would not be captured accurately.
In 1993 seven secondary diagnoses could be given, in 2005 only one.
Further information on the antibodies used is given in Figure 2. Secondary antibodies were Alexa Fluor 488 goat anti-rabbit and anti-mouse IgG (Invitrogen), Alexa Fluor 546 goat anti-mouse IgG (Invitrogen), Dylight 549 goat anti-rabbit IgG (Jackson immuno research), and Cy5 goat anti-rabbit IgG (Millipore).
Details of all primary and secondary antibodies used are given in table 2. Primary and secondary antibodies used for immunohistochemistry, immunofluorescence, and immunoblotting are listed, with inclusion of sources, purpose, and antibody concentrations.
The definition of primary and secondary examinations is given in Table 2. Briefly, an examination is considered to be secondary if in the 48 months preceding the examination there has been a non-negative examination result that could have given rise to the present examination.
Final refinement statistics are given in Table 1. 1) Secondary structural elements, hydrogen bonds, solvent accessible surface area, buried residues, and folding free energy were calculated using VADAR [29].
Primary antibodies used are given in Supplementary Table 2. Additionally, secondary horseradish peroxidase-labelled antibodies (Santa Cruz Biotechnology) were used at dilutions of 1 10000.
Details including the sensitivity analyses are given in Additional file 1. Secondary endpoints for the purpose of characterizing the patient population included renal replacement therapy at any time during hospitalization, survival and major adverse kidney events.
