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High-throughput genotyping technologies allow all of these nsSNPs to be genotyped simultaneously in thousands of patients.
High-density microarrays, which allow many single nucleotide polymorphisms (SNPs) to be genotyped simultaneously in a sample at low cost, have been the technology driving this research.
The system allows 48 SNPs/panel to be genotyped simultaneously in each well for one DNA sample in 96-well or 384-well plate format, with a throughput of 5 10,000 SNPs per hour.
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Five nucleotide variations (nt289, nt793, nt1199, nt1385, nt1623) were genotyped simultaneously in a multiplex assay using oligonucleotides distinguishable by length.
Briefly, each DNA sample was amplified using two multiplex polymerase chain reactions, and the alleles were genotyped simultaneously using an array of immobilized sequence-specific oligonucleotide probes.
We suggest that more markers should be genotyped in subsequent studies for a better mapping.
VH researched the SNPs to be genotyped, prepared samples and was involved in the data analysis.
The broader panel included early passage melanoma cultures that were genotyped in-house (NZM lines) [ 45].
In total, 200 fishish were genotyped and used in QTL analysis (File S1).
In total, 63 polyamine-related SNPs were genotyped, as shown in Supplementary Table S1.
In total, 132 and 131 sires were genotyped in Line 1 and Line 2, respectively.
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