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cDNA will be generated using standard kits and random primers prior to amplification.
Oligopaints robustly label chromosomes both in tissue culture cells and whole-mount tissue preparations and can be generated using standard molecular biology techniques and equipment at a price well below the cost of commercial FISH probes.
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Random sequences were generated using standard C library pseudo-random generator.
The ANN is generated using standard Matlab functions.
Lentiviral constructs were generated using standard molecular biology techniques (Figure 1).
For telomeres in cultured cells, metaphase spreads from cycling MCF-7 cells treated with or without BMP7 (30 ng/mL, 15 h) thee times per week for two weeks were generated using standard laboratory protocols.
Transgenic mice were generated using standard procedures.
Stocks used for overexpression analysis were generated using standard crosses.
Transgenic flies were generated using standard methods [43].
Ad-dnUCP-2 adenovirus was generated using standard procedures [31].
cDNA was generated and cRNA probes were generated using standard protocols.
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