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Synthetic chimeric, or fusion, proteins also may be generated using recombinant DNA technology.
By modifying existing biochemical pathways, or even introducing complete heterologous pathways, a huge array of genotypic and phenotypic variability can be generated using recombinant DNA technology.
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To identify the principal neutralization determinant (PND) of simian immunodeficiency virus (SIV), antisera were generated using recombinant gp110 [the SIV analog of the human immunodeficiency virus type 1 (HIV-1) external envelope glycoprotein, gp120], gp140, several large recombinant and proteolytic envelope fragments, and synthetic peptides of the SIVmac251 isolate.
The samples were then diluted in reagent diluent and standard curves were generated using recombinant proteins (R&D Systems).
The assay measures only the active species of uPA, and a standard curve was generated using recombinant active uPA.
A standard curve was generated using recombinant TNF-α and recombinant IL-10 to express the results in pg/mL.
A mono-nucleosomal template was generated using recombinant Xenopus histones and a 178-bp DNA fragment containing the MMTV nucleosome positioning sequence (NPS) A and an additional 31 bp of DNA to allow an entry point for the polymerase.
In the previous study, two monoclonal antibodies termed V14 and V61 were generated using recombinant sheep PrP as an immunogen to produce glycan-controlled epitopes on the PrP molecule, which discriminate between the two monoglycosylated species [ 16, 17].
Standard curves were generated using recombinant firefly luciferase (Promega) diluted with CCLR to concentrations from 1 pg/ml to 300 ng/ml and a two-phase exponential association curve fitting was performed due to saturation at higher concentrations using the Prism software package (GraphPad Software , Inc San Diego, CA).
An anti-Bi-proVSP antibody was generated using a recombinant Bi-VSP precursor (40-kDa Bi-proVSP) expressed in baculovirus-infected insect cells (Figure S5).
The standard curve was generated using human recombinant VEGF165.
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