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Tolerogenic dendritic cells (tDCs) can be generated in vitro by a variety of methods, including genetic or pharmacological modification.
CD8+ TSCM cells can be generated in vitro from naive CD8+ T cells via Wnt signalling; however, methods do not yet exist for inducing TSCM cells from activated or memory T cells.
Consistent with this notion, we demonstrated previously that high-affinity, peptide-specific TCRs could be generated in vitro by mutations in the CDR3α region of the 2C TCR.
We show that complex structures with features of various committed embryonic tissues can be generated, in vitro, by using early differentiating hES cells and further inducing their differentiation in a supportive 3D environment such as poly(lactic-co-glycolic acid)/poly L-lactic acid) polymer scaffolds.
Indeed, Th cell clones against the fusion proteins TEL/AML1 and BCR/ABL could be generated in vitro [91, 92].
It is worth mentioning that antigen-specific Tr1 cells could be generated in vitro from naïve CD4+ T cells of humans as well [80, 94, 97].
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Imitating the in vivo process, AGEs can be generated in-vitro by incubation of protein solutions with glucose under oxygen atmosphere at 37°C.
Spheroids were generated in vitro in methylcellulose medium.
Moreover, pluripotent stem cell lines were re-derived from the eggs that were generated in vitro, thereby reconstituting the full female germline cycle in a dish.
GAG chitosan complex membranes were generated in vitro and seeded with human ECs or SMCs for culture up to 9 d.
Now a novel class of antibody-like affinity reagents, synthetic antigen binders or sABs, are generated in vitro that target either the open or closed form of maltose-binding protein.
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