For GSK-3-null (double knockout, DKO) cells, more neurospheres were generated in primary culture (in the presence of LIF) but not secondary passages (in the presence of LIF and FGF).
The source analysis results from Experiment 3 indicated that the source of the pure-tone responses was centered on medial Heschl's gyrus, suggesting that the responses were generated in primary auditory cortex.
There is no need for purification because, by taking advantage of the migratory ability of neural crest cells, highly pure populations of hEPI-NCSC are generated in primary culture.
In the current study, the relative rates of SP transplantation versus non-SP transplantation should not be directly compared because very low numbers of SP cells were generated in each primary cell preparation and because comparisons between batches of primary cells were hampered by the toxic effects of Hoechst staining (data not shown).
Assuming that the functional modes are generated in the primary (and/or secondary) areas, Kiebel et al. arguments may well be consistent with the (closely-associated) architecture here exemplified in Scenario 3. Evidence favoring biological realism for the different architectures has been found (or suggested) in various contexts.
However, although fewer T and B cells are generated in the primary lymphoid organs of children with DS, the fate of the differentiating T cells is quite different from that of B cells.
Antibody memory is encoded, in part, in isotype-switched immunoglobulin (Ig G-expressing memory B cells that are generated Ig G-expressingresponse to antigen and give rise to rapid, high-affinity and high-titered antibody responses upon challenge with the samemoryigen.
In our two-step target design, neutrons are generated in a refractory primary target, inducing fission in the surrounding uranium carbide.
Secondary information based detection (SIBD): A secondary information is generated in the result of primary information.
Plasma cells were generated in vitro by differentiation of primary splenic B cells (see Materials and methods and Supplementary Figure S1).
