For example, metabolites may be generated in humans that are absent in rodents or alternatively metabolites may be formed at disproportionately higher levels in humans than in rodents.
Human full-length PEDF was generated in human embryonic kidney cells as described elsewhere, and its purity confirmed using Coomassie and silver staining (Invitrogen).
Infectious AAV particles were generated in human embryo kidney cells at a temperature of 37°C.
Stable clones of human SPL GFP fusion protein or pcDNA 3.0 vector were generated in human embryonic kidney (HEK293T) as described.
Pseudotyped vector particles were generated in human epithelial 293T cells that were co-transfected with pΔenv, pVSV-G and either vEnv-GFP or vGFP, exactly as described [ 15].
Of the four largest data sets currently available, three were generated in human cells, one using labeling [ 2], and two using a tagging approach [ 1, 3], while the fourth data set was generated using protein tagging in yeast [ 4].
For this purpose, extensive experiments are performed and time-course microarray data are generated in human and mouse parenchymal liver cells, human mesenchymal stromal cells and mouse hematopoietic progenitor cells at different time points.
Large TVAs were generated in human (HeLa, Fig. 1B, i) and primate (Vero, Fig. 1B, ii) transformed cell lines, mouse embryonic fibroblasts (MEFs) (Fig. 1B, iii) and primary mouse dendritic cells (Fig. 1B, iv).
Lentiviruses expressing a short hairpin RNA (shRNA) construct for silencing IL-6 (Vector Core, University of Michigan) were generated in human embryonic kidney cells (293 T) transfected by the calcium phosphate method, as described [ 25].
