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Human pluripotent stem cells (hPSC) can be isolated from in vitro fertilized eggs or they can be generated from somatic cells through the process referred to as re-programming[1, 2].
Induced pluripotent stem (iPS) cells can be generated from somatic cells by coexpression of four transcription factors: Sox2, Oct4, Klf4, and c-Myc.
iPSCs can be generated from somatic cells of any individual and these pluripotent cells can be differentiated into the desired cell type.
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Neoantigens are generated from somatic point mutations in tumor tissues that are absent in normal tissue.
As mentioned above, a SAGE library has been generated from somatic tissues of adult hermaphrodites [ 24].
Shoot buds were collected on May 4 and 6, 2009, from the second and third whorls of branches of 9-year-old Picea glauca (white spruce) trees that were generated from somatic embryos as previously described [ 9].
The most comprehensive study of transcript profiling in Pinus embryos has been conducted in P. taeda, where approximately 68,700 ESTs have been generated from somatic and zygotic embryos [ 9].
Then we assessed whether ciNPCs could be generated from different somatic cells or cells from different species with similar strategies.
Recently, Houet et al. [28] showed that pluripotent stem cells can be generated from mouse somatic cells at an efficiency of 0.2% by using a combination of seven small-molecule compounds.
These induced pluripotent stem cells (iPSCs) can be generated from different somatic cell sources by overexpression of specific transcription factors (TF) (Takahashi & Yamanaka, 2006; Yu et al, 2007) and may open a new chapter for the field of regenerative medicine.
The discovery of induced pluripotent stem cell (iPSC) technology has raised hopes in circumventing the current limitations in cell-based therapies where autologous stem cells could be generated from terminally differentiated somatic cells.
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