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Due to the huge number of slices that can be generated from single range image, even a simple description model of slice can achieve robust performance.
Colonies could be generated from single cells from all four NS cell populations.
Hybrid approaches combining high-throughput short-read assembly with strategic approaches that enable physical or virtual binning of reads into haplotypes are enabling multi-gene haplotypes to be generated from single individuals.
Because obtaining multiple biopsies from the same patient will likely be the exception, in most clinical trials the majority of gene expression profiles will have to be generated from single core biopsies that are likely to yield these small amounts of starting RNA material.
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In order to obtain high quality full-length cDNA sequences, clones were selected where the full-length cDNA sequences could be generated from single-pass sequencing and re-sequencing of individual clones, not from contig assemblies.
RT was carried out as previously described cDNA was generated from single cells based on the Smart-seq2 method as described previously51; 0.1 µl 100 µl Oligo-dT (5′-AAGCAGTGGTATCAACGCAGAGTACT30VN-3′ 5′-AAGCAGTGGTATCAACGCAGAGTACT30VN-3′ 5′-AAGCAGTGGTATCAACGCAGAGTACT30VN-3′ermoFisher) and 2.8 µl of trehalose (1.0) were added to each sample.
Building on the hanging-drop concept, mEB have also been generated from single cell suspensions of mESC in microcentrifuge tubes [14], and in 96-well U-bottom microtiter plates [16].
All cells were cultured in DMEM high glucose (Hyclon, Logan, Utah) supplemented with 10% FBS and 1% penicillin/streptomycin. MDA-MB-435-GFP derivative cell lines were generated from single cell cloning through initial seeding of single cells in 96-well plates and subsequent expansion of cell numbers.
SAS were generated from single mycobacterial clinical isolates.
These computational derived consensus assemblies containing open reading frames (ORFs) are generated from single pass reads through cDNA libraries.
We found no difference in the intensity of Gadd45g-d4Venus between sister cells that were generated from single INPVZ cells (14 of 14 apically generated pairs).
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