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As genetically engineered tumor vaccines, tumor liposomes have the potential to be generated from primary tumor tissue isolated from individual patients, providing a vaccine that is specialized for each individual.
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In plants, miRNAs are generated from primary miRNA transcripts (pri-miRNAs) that are generally transcribed by RNA polymerase II and fold back on themselves to form distinctive hairpin structures [ 1].
The MDA-IBC-3 cell line was generated from primary human breast cancer cells isolated from pleural effusion fluid obtained on a clinical protocol approved by the institutional review board from a patient with inflammatory breast cancer (IBC).
Four hiPSC lines, UTA-SF2-1, UTA-SF2-2, UTandF3-1, and UTA-SF3-2, were generated from primary adult human dermal fibroblasts (female of 41, 46, or 51 yr old; 106-05a, cell applications, inc. San Diego, CA) under hESF9a-based conditions.
Single-cell suspensions (>95% single cells) were generated from primary mammary tumors as described earlier.
Mature miRNAs are generated from primary miRNAs (pri-miRNAs) via two steps.
Bone marrow- (BMDM) and monocyte-derived macrophages (MDM) were generated from primary monocytes.
It had an added benefit of being generated from primary keratinocytes (NHEK) and more importantly conforming to ENCODE guidelines [ 29].
In healthy individuals, GCs are generated from primary B-cell follicles of secondary lymphoid organs during T-cell-dependent immune responses [ 56].
The mouse xenograft models are generated from primary tumors, metastases, and "cancer stem cells" derived from both primary and metastatic tissue samples.
Composite variables were generated from primary responses using the egen command in stata to constitute our primary outcome variables of interest.
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