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TR cells can be generated from peripheral CD4+CD25- T cells, but only in response to activation.
Originally considered an exclusive product of the thymus, important new data indicate that these cells can be generated from peripheral CD4+ T cells and expanded for delivery as a cellular therapeutic strategy.
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DCs were generated from peripheral blood mononuclear cells of healthy donors.
DCs were generated from peripheral blood mononuclear cells (PBMC).
DC were generated from peripheral blood monocytes as described before [21].
CD172+ cells were generated from peripheral blood mononuclear cells (PBMCs) and were cultured in the presence of rpGM-CSF and rpIL-4.
Late-EPCs were generated from peripheral blood mononuclear cells (PBMCs) of cKS patients according to methods described by Ingram [9], with minor modifications.
Since MBP-clones were generated from peripheral blood cells that already contain native peripheral CD25+FoxP3+ cells, their increased numbers in PP14·Fcγ1-treated wells can result either from expansion of the native Treg population or due to the emergence of adaptive FoxP3+ T cells from naïve cell population.
Germline data were generated from peripheral blood samples.
CIK cells were generated from peripheral blood mononuclear cells of three healthy volunteers.
Dendritic cells were generated from peripheral blood, and cultured autologous glioma cells were established from surgical specimens in each case.
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