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Few datasets have been generated for stroma, and this is the first extensive dataset to be generated from normal stroma.
Thyroid carcinomas are thought to be generated from normal thyroid follicular cells (thyrocytes) by multi-step carcinogenesis (Farid et al, 1994).
Secondly, such Mabs can be generated from normal, non tumour bearing subjects at a frequency comparable to their production from tumour patients.
This latter observation is true also for common autoantigens such as DNA and IgG since Mabs to these can also be generated from normal subjects in addition to autoimmune individuals.
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METHODS: Autologous EBV-transformed B-lymphoblastoid cell lines (BLCLs) were generated from normal donors, and CTLs were initiated by culturing peripheral blood mononuclear cells with DCs alone, disrupted BLCLs alone, intact, irradiated BLCLs alone, and DCs loaded with disrupted BLCLs.
The iPS cells were generated from normal human fibroblast cultures as described previously (Yu et al., 2007) with lentiviral vectors expressing OCT4, NANOG, LIN28 and SOX2.
3'-RACE libraries were generated from normal, BPH, PCa, and metastatic PCa RNA with Superscript III (Invitrogen: Cat No. 18080-093) using primers and protocols described in the SMART RACE User Manual (BD Biosciences Clontech).
Human monocyte-derived DC (MD-DC) were generated from normal volunteers' monocytes purified after elutriation of peripheral blood according to the French EFS procedures (Pr J. Bernard, Institut Jean Godinot, Reims, France).
Our results and recommendations are based on extensive simulation studies from data which are generated from normal distributions.
Human MDMs were generated from normal donors [ 16] and infected with HIV-1BaL as described previously [ 12, 13].
Follicular carcinomas are generated from follicular adenomas, while papillary carcinomas are derived from some unknown precursor cells that are generated from normal thyrocytes.
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