Despite the similarity in protein structures, the binding profiles of each protein varied significantly, suggesting that differential fluorescence responses would be generated for each FP upon interaction of the sensor with competing analytes such as mammalian cells.
The necessary spatial scale separation could be generated, for example, through preferential allosteric interactions of the PFK effectors with immobilized enzymes [ 32, 63], including the PFK itself.
Furthermore, the direct multiplication of the proposed formulas on the P M interaction diagram for a short RC column subjected to axial force and a uniaxial bending moment enables a P M interaction diagram to be generated for a slender RC column subjected to long-term axial force and biaxial bending moments.
The interaction map was generated for the interaction of enriched pathways using CytoScape software.
The interaction map was generated for the interaction of enriched transcription factors and their putative target genes using CytoScape software One microgram of total RNA was reverse transcripted using random hexamers and superscript -II reverse transcriptase (Invitrogen, Carlsbad, CA).
The response surface and contour plots were generated for different interaction of any two independent variables, while holding the value of third variable as constant at the central level.
These values were generated for homologous interactions where αAwt interacts with C-terminal truncated αA-crystallins and also for heterologous interactions where αBwt interacts with C-terminal truncated αA-crystallins (Fig. 8) As expected, negative control (vectors alone) showed very little interaction while positive controls (αAwt/αAwt and αBwt/αAwt) showed significant interaction.
The advantage of the classic matching algorithm is that it performs considerably faster than the secondary match algorithm in cases where a large number of ligand placements have been generated for preceding interactions of the theozyme.
A significant amount of protein interaction data has been generated for Arabidopsis, but the interactions have been produced using different analysis methods and stored in different databases (12).
Results were generated for three levels of interaction.
