To minimize the effect of fusion on enzymatic activity, six fusion proteins were generated by attaching the interacting proteins to the C or N terminus of cellulases.
A peptide affinity resin was generated by attaching the peptide to Sulpho-Link resin (Pierce) via the N-terminal cysteine after treatment of the peptide with immobilized TCEP disulfide reducing gel (Pierce).
Neutral surfaces were generated by attaching the appropriate number of H+ ions in a variety of different ways.
The affinity resin was generated by attaching N-[ 6-aminohexyl carbamoylmethyl]-NAD to a CN-[ 6-aminohexyl carbamoylmethyl]-NADatrix (Amersham Pharmacia Biotech).
Mouse (Song et al., 2004) and rabbit (Pattison et al., 2008) models overexpressing PLN in slow-twitch (type I) muscle fibers (PlnOE) have been generated by attaching a Pln transgene to the β-myosin heavy chain (β-MHC) promoter, which preferentially directs high levels of expression of type I skeletal-muscle-specific transgenes (Rindt et al., 1993, 1995; Knotts et al., 1994).
A small amount of electrical power, typically in the micro-watt or milli-watt range, can be generated by a thermoelectric module attached to the outside of the computer's standard heat sink assembly, when a secondary heat sink is attached to the other side of the thermoelectric module.
The allocation sequence will be generated by the research assistant attached to the trial.
If appropriate electrical contacts are attached to a nanotube, electron-hole pairs (excitons) can be generated by injecting electrons and holes from the contacts.
(The films were generated by nine cameras attached to a grid mounted on the bonnet of an assistant's slow-moving car, and were "directed" by the artist in the back seat, using a grid of nine monitors to draw and edit the unfolding landscape).The colours are bright, the strokes bold.
The circulation within the flow chamber was generated by an externally attached electric motor, which rotates up to 500 rounds per minute (rpm).
