Additional CrChlI constructs were generated by deleting various sequences from 35S:irintCrChlI by digestion with appropriate restriction enzymes followed by religation of the plasmid, or were generated as intermediates during the cloning of the final 35S:irintCrChlI construct.
A small quantity of monochloramine and dichloroamine were generated as intermediate oxidation products in the process, indicating that ammonia was chlorinated in a stepwise process and finally converted into nitrogen.
Another possibility is that the half-tRNA molecules may be generated as splicing intermediate molecules from intron-containing tRNAs.
In addition to mitochondrial sources of O2–, this reactive intermediate can be generated as a byproduct during the catalytic cycle of numerous enzymes, such as "nonspecific" peroxidases (i.e., haem-containing peroxidases capable of using H2O2 to oxidize a range of substrates), as well as xanthine and aldehyde oxidases.
The method would need to start with a different precursor, such as a sulfonyl chloride, and rely upon an [18F]sulfonyl fluoride being generated as an intermediate, to be potentially applicable as a n.c.a.
This finding suggests a stepwise histone demethylation after the loss of expression of the targeted methyltransferase, in which H3K9me2 is generated as an intermediate by demethylation of H3K9me3.
These were generated as an intermediate step in producing cohort life tables 3 by first estimating life expectancies at birth by fitting logistic curves to expectancies, by sex, from the Rungpitarangsi and SPC tables, then obtaining nqx-values (age-specific probabilities of dying) from Coale and Demeny's (1983) 'West' model life tables.
The DFCC 1 is generated as a transient intermediate near the hypothetical branching point of the PaO/phyllobilin pathway at which the type-II phyllobilins diverge from the first formed 1-formyl-19-oxobilin-type Chl catabolites (or type-I phyllobilins).
The strand-displacement model of mtDNA replication [2,8] was suggested to be based on the partially single-stranded replication intermediates that are generated as a result of ribonucleotide loss from the replication intermediates of RITOLS replication [4,6,7].
