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Given the above findings, we hypothesized that maternal diabetes adversely impacts the mitochondria in cumulus cells, which may be further transferred into the oocyte, contributing to poor oocyte quality.
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The compound was further transferred into FSW to make up the following test solution with the concentrations ranging from 25 to 0.50 μg ml−1.
Then, the obtained clear liquid was sonicated in an ultrasonic for 2 h, resulting in the formation of a milk-like solution which was further transferred into a stainless autoclave and kept at 453 K for 13 h.
Next, the pattern was further transferred into the silicon wafer with Al as mask using Oxford Instruments ICP380 dry etching system (Abingdon, UK) with C4F8 and SF6 gases [10], followed by Al removal process.
These plants were further transferred into pots containing vermiculite and grown under normal condition for 8 d and 21 d.
The seedlings under 150 mM NaCl treatment were further transferred into soil and grown for two weeks under normal conditions.
This pattern was further transferred into the polyimide by a directional oxygen reactive-ion etch.
The upper aqueous phase was further transferred into another centrifuge tube and 500 μL of isopropanol added to each sample.
The resulting constructs (pENTRY-ZmTCRR2, pENTRY-ZmHP1,2 and 3 were further transferred into the bacterial expression vectors pDEST15 and pDEST17 (Invitrogen) for N-terminal GST-tagging or His-tagging, respectively.
One hundred eighty cDNA sequences with 100% sequence accuracy were further transferred, by in vitro DNA recombination, into two different destination vectors with either FLAG or GFP as the C-terminal fusion tags.
It was then further transferred into 900.0 ml medium and incubated at 30°C up to 07 days.
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